A high-throughput neutralizing antibody assay for COVID-19 analysis and vaccine analysis
Virus neutralization stays the gold commonplace for figuring out antibody efficacy. Due to this fact, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently wanted for COVID-19 serodiagnosis, convalescent plasma remedy, and vaccine improvement.
Right here, we report on a fluorescence-based SARS-CoV-2 neutralization assay that detects SARS-CoV-2 neutralizing antibodies in COVID-19 affected person specimens and yields comparable outcomes to plaque discount neutralizing assay, the gold commonplace of serological testing.
The fluorescence-based neutralization assay is particular to measure COVID-19 neutralizing antibodies with out cross reacting with affected person specimens with different viral, bacterial, or parasitic infections. Collectively, our method affords a speedy platform that may be scaled to display screen folks for antibody safety from COVID-19, a key parameter essential to securely reopen native communities.
[Phase Ⅲ randomized controlled, multicenter, prospective study of recombinant anti-HER2 humanized monoclonal antibody (Cipterbin) combined with vinorelbine in patients with HER2 positive metastatic breast cancer: the HOPES Study]
Goal: To guage the scientific efficacy and security of recombinant anti-HER2 humanized monoclonal antibody (Cipterbin) mixed with vinorelbine in sufferers with HER2 constructive metastatic breast most cancers.
Strategies: Sufferers have been randomized 2∶1 to check group and management group. Sufferers in take a look at group obtained Cipterbin (four mg/kg loading dose and a couple of mg/kg upkeep dose every week, IV) mixed with vinorelbine (25 mg/m(2) on days 1,eight and 15 of every 28 days, IV). Sufferers in management group obtained vinorelbine (25 mg/m(2) on days 1,eight and 15 of every 28 days, IV).The first finish level was development free survival (PFS).
Outcomes: A complete of 315 sufferers have been enrolled from Jan 2009 to Jan 2013 (212 in take a look at group and 103 in management group). The median PFS of take a look at group was considerably longer than that of management group, 39.1 weeks vs 14.Zero weeks (HR=0.24; 95%CI, 0.16-0.36; P<P<P<Conclusion: The recombinant anti-HER2 humanized monoclonal antibody (Cipterbin) mixed with vinorelbine has important efficacy and good security.
It’s the optimized remedy regime for sufferers with taxane-pretreated HER2 constructive metastatic breast most cancers, which gives extra focused remedy alternatives for HER2 constructive breast most cancers sufferers in China.
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Galectin 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This monoclonal antibody is for studies of antigen expression in cells and tissue sections using immunocytochemistry and immunoprecipitation
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Galectin-1 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells. The Galectins are lectins that recognize and interact with β-galactoside moieties. Galectin-1 is an animal lectin that has been shown to interact with CD3, CD4, and CD45. It induces apoptosis of activated T-cells and T-leukemia cell lines and inhibits the protein phosphatase activity of CD45. Recombinant human Galectin-1 is a 14.5 kDa protein containing 134 amino acid residues.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Galectin 1 (GAL1) in serum, plasma and other biological fluids.
Description: Quantitativesandwich ELISA kit for measuring Human Galectin-1 (LGALS1) in samples from serum, plasma, tissue homogenates, cell lysates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Galectin-1(LGALS1) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Galectin-1(LGALS1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Galectin-1 (Gal-1) Antibody (Biotin Conjugate)
Description: A sandwich quantitative ELISA assay kit for detection of Human Galectin 1 (GAL1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Galectin 1 (GAL1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Galectin 1 (GAL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Galectin 1 (GAL1) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creatingimmune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Description: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Description: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.
Description: Recombinant Human Galectin-9(LGALS9) expressed in E.coli
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Drug Tolerant Anti-drug Antibody Assay for Infliximab Remedy in Scientific Apply Identifies Constructive Circumstances Earlier
A subgroup of sufferers handled with infliximab lose response to the therapy and one purpose for that is the event of anti-drug antibodies (ADA). If used optimally, measuring drug and ADA degree might result in a extra personalised and environment friendly therapy regime, and allow identification of ADA-positive sufferers earlier than the underlying illness flares or allergic reactions happen.
With using a drug-tolerant ADA assay which might detect ADA regardless of drug ranges within the pattern, we decided the influence of ADA on therapy failure to infliximab. The goals of this research have been to estimate the real-life optimum serum infliximab (sIFX) degree and set a scientific threshold worth for a drug-tolerant ADA assay.
Trough ranges of sIFX have been measured with ELISA. Free ADA was measured with two drug-sensitive strategies (ELISA and a bioassay) and one drug-tolerant technique (PandA). Two real-life cohorts handled with infliximab have been included; a cross-sectional cohort together with sufferers with inflammatory rheumatic illnesses (n = 270) and a potential cohort of rheumatoid arthritis (RA) sufferers (n = 73) adopted for 1 yr.
The conventional vary of sIFX was estimated from the potential cohort and an arbitrary optimum drug degree was set to be between 1 and 6 μg/mL. Utilizing this vary, optimum sIFX was present in solely 60% (163/270) of the sufferers within the cross-sectional cohort. These sufferers had considerably higher therapy response than these with a drug degree below 1 μg/mL, who had an ADA frequency of 34% (19/56) utilizing the drug-tolerant technique.
Within the potential cohort, the drug-tolerant assay might establish 34% (53/155 samples) as ADA constructive in samples with sIFX degree >0.2 μg/mL. ADA have been seldom detected in sufferers with >1 μg/mL sIFX, with three attention-grabbing exceptions. A clinically related ADA threshold was decided to be >three RECL as measured with the drug-tolerant assay.
In a real-life setting, there was a considerable variety of sufferers with suboptimal drug ranges and a proportion of those had ADA. Each too low and too excessive drug ranges correlated with worse illness, however for various causes. Including a drug-tolerant assay enabled detection of ADA earlier and no matter drug degree at time of sampling.
Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Integrin alpha-5 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5 (ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid (CSF). A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Integrin alpha-5(ITGA5) in samples from serum, plasma, cell culture supernates, urine, cerebrospinalfluid(CSF). Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Integrin Alpha 5 (ITGa5) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Integrin Alpha 5 (ITGa5) in Tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Integrin Alpha 5 (ITGa5) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].
Description: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [RefSeq]
Mouse Anti Human Integrin Alpha V Beta 5 Monoclonal Antibody
Description: A sandwich ELISA kit for detection of Integrin Alpha 5 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: Integrin alpha-5 belongs to the Integrin alpha chain family and contains 7 FG-GAP repeats. Integrin alpha-5 joins with Integrin- beta 1 to form a fibronectin and laminin receptor which recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. It is expressed on fibroblasts, endothelial cells, peripheral T cells and platelets. Integrin alpha-5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains. In addition to adhesion, ITGA5 participates in cell-surface mediated signalling.
